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PCR-tests in cats with plasmacytic pododermatitis
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Plasmacytic pododermatitis is an unique disease of cats whose pathogenesis is not fully clear. In this study, DNA of various infectious agents (including bacteria, viruses and toxoplasma) were tested via PCR. Very interesting!
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Sections of 14 skin biopsies of cats with plasmacytic pododermatitis and a clinical follow-up of 12–36 months were stained with a polyclonal anti-Mycobacterium bovis (Bacille Calmette-Guerin = BCG) antibody cross-reactive to a broad spectrum of fungi and bacteria.
All sections were negative for organisms within the actual footpad tissue with the anti-BCG antibody stains.
Polymerase chain reaction (PCR) assays that amplify the DNA of Bartonella spp., Ehrlichia spp., Anaplasma phagocytophilum, Chlamydophila felis, Mycoplasma spp., Toxoplasma gondii, and feline herpesvirus 1 (FHV-1) were applied to tissue digests. DNA of those pathogens assessed was not amplified from tissue.
Source: S. V. Bettenay, M. R. Lappin and R. S. Mueller (2007): An Immunohistochemical and Polymerase Chain Reaction Evaluation of Feline Plasmacytic Pododermatitis. In: Vet Pathol 44:80-83 (2007)
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