Cultured 9L cells were incubated with varying concentrations of pheophorbide-a-hexyl ether (HPPH) and then exposed to 665-nm red light from a non-coherent light source or a dye laser.
Cell death was produced by both light sources, with the non-coherent light being most effective at the highest HPPH concentrations. To assess the feasibility of using the non-coherent light source for clinical photodynamic therapy (PDT), four dogs and three cats presenting with spontaneous superficial tumours were injected intravenously with 0.15 mg kg1 of HPPH, 1 h before their tumours were irradiated with 665-nm non-coherent light (50 mW cm2, 100 J cm2).
Of the nine tumours treated, there were eight complete responses, all occurring in animals with squamous cell carcinoma. After 68 weeks of follow-up, the median initial disease-free interval had not been reached.
These data suggest that non-coherent light sources may be efficacious for photodynamic therapy of spontaneous superficial tumours in animals, representing a cost-effective alternative to medical lasers in both veterinary and human oncology.
Source: Reeds, K. B., Ridgway, T. D., Higbee, R. G. & Lucroy, M. D. (2004): Non-coherent light for photodynamic therapy of superficial tumours in animals. In: Veterinary and Comparative Oncology 2 (3), 157-163.
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