The horses were sedated intravenously with detomidine hydrochloride (0.015 mg/kg). The pupil was dilated and the auriculopalpebral nerve was blocked. The ERGs were recorded with the active electrode on the cornea (DTL), the reference electrode near the lateral canthus, and the ground electrode over the occipital bone. The light intensities of the white strobe light were 0.03 cd·s/m2 (scotopic) and 3 cd·s/m2 (scotopic and photopic). Photopic and scotopic single flash and flicker responses to Ganzfeld stimulation were recorded. During the 20-min dark adaptation period the retina was stimulated every 5 min with the 0.03 cd·s/m2 single flash.
The median b-wave amplitudes and implicit times were 38 µV and 33 ms (photopic cone-dominated response), 43 µV and 63 ms (5-min dark adaptation), 72 µV and 89 ms (10 min), 147 µV and 103 ms (15 min), 188 µV and 109 ms (20 min, 0.03 cd·s/m2, rod response), and 186 µV and 77 ms (20 min, 3 cd·s/m2, maximal combined rod-cone response). A steady increase in amplitude and implicit time was noted during dark adaptation. No oscillatory potentials could be isolated.
The use of detomidine hydrochloride sedation and the DTL microfiber electrode allowed the recording of good quality ERGs. This protocol should permit the detection of functional problems in the retina without the risk involved with general anesthesia.
Source: Komáromy, A.M., Andrew, S.E., Sapp, H. L., Brooks, D.E., Dawson, W.W. (2003): Flash electroretinography in standing horses using the DTL microfiber electrode.
In: Veterinary Ophthalmology 6 (1), 27-33.
www.blackwell-synergy.com/links/doi: 10.1046/
j.1463-5224.2003.00261.x
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